Trippier was sent off when referee Bobby Madley awarded a penalty against him for deliberate hand ball on the line with the club appealing the decision yesterday.
However, the FA have supported the referee and upheld the decision which means Trippier must now serve a one match ban this Saturday when we travel to the KC Stadium.
For Tripps, it brings to an end a run of 63 consecutive league appearances for the Clarets since he signed, initially on loan, from Manchester City in the summer of 2011.
He started all 46 games last season and having been sent off at Leeds he missed the FA Cup tie at Norwich before returning for the next league game at Middlesbrough.
The one match ban remains which means he'll be available for the trip to Barnsley next Tuesday to face the team where he spent the 2010/11 season on loan.